Plasmid

Part:BBa_K4869000:Design

Designed by: Woo Rin Lee   Group: iGEM23_Korea-HS   (2023-09-08)


BII-CMV-AfmW3A piggyBac vector contains Afamin and Wnt3a genes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7374
    Illegal XbaI site found at 923
    Illegal XbaI site found at 6885
    Illegal SpeI site found at 917
    Illegal SpeI site found at 1813
    Illegal SpeI site found at 6637
    Illegal PstI site found at 1129
    Illegal PstI site found at 1171
    Illegal PstI site found at 2979
    Illegal PstI site found at 3106
    Illegal PstI site found at 3756
    Illegal PstI site found at 3891
    Illegal PstI site found at 4137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7374
    Illegal NheI site found at 889
    Illegal SpeI site found at 917
    Illegal SpeI site found at 1813
    Illegal SpeI site found at 6637
    Illegal PstI site found at 1129
    Illegal PstI site found at 1171
    Illegal PstI site found at 2979
    Illegal PstI site found at 3106
    Illegal PstI site found at 3756
    Illegal PstI site found at 3891
    Illegal PstI site found at 4137
    Illegal NotI site found at 3971
    Illegal NotI site found at 4494
    Illegal NotI site found at 4661
    Illegal NotI site found at 4805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7374
    Illegal BglII site found at 872
    Illegal BamHI site found at 911
    Illegal BamHI site found at 2253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7374
    Illegal XbaI site found at 923
    Illegal XbaI site found at 6885
    Illegal SpeI site found at 917
    Illegal SpeI site found at 1813
    Illegal SpeI site found at 6637
    Illegal PstI site found at 1129
    Illegal PstI site found at 1171
    Illegal PstI site found at 2979
    Illegal PstI site found at 3106
    Illegal PstI site found at 3756
    Illegal PstI site found at 3891
    Illegal PstI site found at 4137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7374
    Illegal XbaI site found at 923
    Illegal XbaI site found at 6885
    Illegal SpeI site found at 917
    Illegal SpeI site found at 1813
    Illegal SpeI site found at 6637
    Illegal PstI site found at 1129
    Illegal PstI site found at 1171
    Illegal PstI site found at 2979
    Illegal PstI site found at 3106
    Illegal PstI site found at 3756
    Illegal PstI site found at 3891
    Illegal PstI site found at 4137
    Illegal NgoMIV site found at 1044
    Illegal NgoMIV site found at 2176
    Illegal NgoMIV site found at 6537
    Illegal NgoMIV site found at 7006
    Illegal NgoMIV site found at 7258
    Illegal NgoMIV site found at 8031
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3168
    Illegal BsaI site found at 9207
    Illegal BsaI.rc site found at 4486
    Illegal BsaI.rc site found at 5879
    Illegal SapI.rc site found at 10289


Design Notes

This project aimed to generate Afamin-Wnt3a producing cells to obtain FBS-free growth medium for cultivating bovine muscle cells.


Source

Mus mucus and Homo Sapiens genes are inserted.

References